Imtakt chromatographic column​-Unison UK-C18 Column Details
Unison UK-C18
Characteristics of chromatographic column
◇ Higher column efficiency

◇ Lower column pressure
◇ More suitable for separating hydrophobic compounds
◇ Multiple fixed phase options
◇ Can use 100% pure water mobile phase

Analyzing polar compounds with high resolution
Quick detection
By utilizing the high column efficiency and short column length of the Unison UK-C18 chromatographic column, samples can be rapidly and massively detected. Many users have significantly reduced the analysis time and achieved satisfactory separation using chromatography columns with lengths of 10mm, 20mm, and 30mm. All lengths of chromatography columns are packed with 3 μ m silica gel. When users are searching for optimal conditions between analysis speed and separation, it is recommended to use the Unison UK-C18 chromatographic column.
Excellent resolution in acidic eluent
For liquid chromatography-mass spectrometry technology, formic acid is an indispensable substance. The Unison UK-C18 chromatographic column stationary phase showed excellent stability even after soaking in a 0.1% formic acid solution for 170 hours.
Avoid collapse of fixed phase
The traditional ODS silica gel stationary phase designed for separating polar compounds typically has lower coverage compared to silica gel stationary phase. This is very important for avoiding the collapse of fixed phases.
The Unison chromatographic column provides a low coverage ODS stationary phase due to its three functional components and unique capping technology, which allows the stationary phase of the chromatographic column to remain stable even in aqueous mobile phases with a pH of 1.5.
The traditional ODS chromatography column shows a C18 stationary phase collapse, resulting in reduced retention time, poor separation efficiency, and reproducibility. The Unison UK-C18 chromatographic column can avoid the collapse of the stationary phase like ordinary ODS chromatographic columns. Even when conducting nucleic acid analysis under 100% pure water conditions, there will be no collapse of the chromatographic column caused by loss of the stationary phase.
pHWide range of direct sales and high durability
Unison UK-C18 chromatographic column stationary phase can maintain high stability and durability in both acidic and alkaline solutions.
The unique sealing technology of Unison UK series chromatography columns ensures that C8 and phenyl chromatography column stationary phases are suitable for a wide pH range and have a high service life under acidic or alkaline conditions, just like Unison UK-C18 chromatography column stationary phases.
Outstanding strong acid stability
Research has shown that under acidic conditions of pH 1.5, traditional ODS chromatography columns with trimethylsilyl capped stationary phases exhibit strong stability. However, the Unison UK-C18 chromatographic column has stronger stability. The unique sealing technology of Unison UK-C18 chromatography column is an excellent choice for separating hydrophilic compounds under acidic conditions.
Under acidic conditions of pH 1.5, the Unison UK-C18 chromatographic column exhibits stronger stability compared to traditional ODS columns.
Analyzing organic acids under low pH conditions
The reaction separation of organic acids is very difficult. The Unison UK-C18 chromatographic column can provide excellent separation efficiency and peak shape.
Usually, the separation of organic acids requires a 250mm long chromatographic column, but a 150mm long Unison UK-C18 chromatographic column can achieve the same separation effect.
Separate polar compounds
Under hydrophilic conditions, the analysis of nucleic acid substances, especially adenine, using a common commercially available ODS chromatographic column often results in poor chromatographic peak symmetry. Acidic eluents are typically used to analyze water-soluble vitamins in the absence of ion pairing reagents, which are standard high polarity compounds. Rapid separation can be achieved using any Unison chromatographic column.
Separation of vitamins
The analysis of water-soluble vitamins is very important and requires an aqueous mobile phase under acidic conditions to achieve excellent separation efficiency.
Compared to the traditional 5 μ m, 150mm long column and 3.5 μ m particle size, the Unison UK-C18 column using phosphoric acid mobile phase provides excellent peak shape and rapid separation.
Unique sealing technology
UnisonThe chromatographic column produced using unique capping technology exhibits excellent elution performance for difficult to separate substances such as alkaline compounds. This technology is not only applied to Unison UK-C18 chromatography columns, but also to Unison's C8 and phenyl chromatography columns.
For alkaline antidepressants with pKa values analyzed, all stationary phases of Unison exhibited excellent peak shapes, even when using ammonium acetate as a variable pH regulator for liquid chromatography-mass spectrometry. This means that separation can be achieved even without using phosphoric acid eluent, which is commonly used for UV detection in liquid chromatography column analysis. This can help you avoid the trouble caused by using inorganic salts.
Imtakt chromatographic column​-Unison UK-C18 Column Details

analytical column

Semi prepared column

Protect the column core, protect the column card sleeve with chromatographic connectors